Original Article

Combined Radiofrequency and Ultrasonic Cavitation Therapy Does not Have Adverse Effects on Hematological and Liver Markers in Overweight Women


Background: Although dietary management is the principle intervention for treating obesity, noninvasive body-contouring methods are also gaining currency. We aimed to explore the effects of combined radiofrequency (RF) and ultrasound (US) body contouring on anthropometric indices, hematological markers, and anti-HSP27 levels in overweight females.
Methods: In this randomized control clinical trial, fifty overweight females were enrolled and divided into two groups. Each participant was prescribed a diet with a daily calorie deficit of 500 kcal. RF and US were each used once a week for 5 weeks in the intervention group. Anthropometric and hematological markers were measured in all subjects before and after the intervention.
Results: Abdominal circumference (AC), waist circumference (WC), body mass index, and body fat mass were reduced significantly in both groups (p < 0.001). However, the mean reduction in AC (p < 0.05) and WC (p < 0.001) were significantly greater in the intervention group compared with the control group. Moreover, levels of WBC, HCT, Hb, MCV, and MCH were significantly reduced after the RF-US intervention, although all the changes were in the normal range, suggesting that this therapy did not have adverse effects on hematological parameters. Also, the level of anti-HSP27 did not show any significant change.
Conclusion: Our study suggests that body-contouring devices based on RF and US cavitation reduce measures of adiposity and do not have adverse effects on hematological factors, liver function markers, and HSP27 level in overweight women. Further investigations are required to explore the value of this method in a larger multicenter setting.

Mandviwala, T., U. Khalid, and A. Deswal, Obesity and cardiovascular disease: a risk factor or a risk marker? Current atherosclerosis reports, 2016. 18(5): p. 1-10.

Onorati, F., et al., Impact of endothelial activation on infective and inflammatory complications after cardiac surgery in type II diabetes mellitus. The International journal of artificial organs, 2011. 34(6): p. 469-480.

Control, C.f.D. and Prevention, National diabetes fact sheet: national estimates and general information on diabetes and prediabetes in the United States, 2011. Atlanta, GA: US Department of Health and Human Services, Centers for Disease Control and Prevention, 2011. 201(1).

Krueger, N. and N.S. Sadick, New-generation radiofrequency technology. Cutis, 2013. 91(1): p. 39-46.

Weiss, R.A. Noninvasive radio frequency for skin tightening and body contouring. in Seminars in cutaneous medicine and surgery. 2013. Frontline Medical Communications.

Mulholland, S., Clinics In Plastic Surgery Journal 2011.

Gogate, P.R., R.K. Tayal, and A.B. Pandit, Cavitation: a technology on the horizon. Current Science, 2006. 91(1): p. 35-46.

Prisco, M.G., et al., Effects of GSM-modulated radiofrequency electromagnetic fields on mouse bone marrow cells. Radiation research, 2008. 170(6): p. 803-810.

Saedi, N. and M. Kaminer. New waves for fat reduction: high-intensity focused ultrasound. in Seminars in cutaneous medicine and surgery. 2013. Frontline Medical Communications.

Ghayour-Mobarhan, M., et al., Antibody titres to heat shock protein 27 are elevated in patients with acute coronary syndrome. Int J Exp Pathol, 2008. 89(3): p. 209-15.

Nowrousian, M.R., Recombinant Human Erythropoietin (rhEPO) in clinical oncology: Scientific and clinical aspects of anemia in cancer. 2007: Springer Science & Business Media.

Jewell, M.L., et al., Safety and tolerability of high-intensity focused ultrasonography for noninvasive body sculpting: 24-week data from a randomized, sham-controlled study. Aesthetic Surgery Journal, 2012. 32(7): p. 868-876.

Takegami, K., et al., Erythrocytes, as well as microbubble contrast agents, are important factors in improving thermal and therapeutic effects of high-intensity focused ultrasound. Ultrasound in medicine & biology, 2005. 31(3): p. 385-390.

Leal, D.H., Combined Modality of Focused Ultrasound and Radio-Frequency for Non-Invasive Fat Disruption and Body Contouring – Results of a Single Treatment Session, 2010.

Tavallaie, S., et al., Association between indices of body mass and antibody titers to heat-shock protein-27 in healthy subjects. Clinical biochemistry, 2012. 45(1): p. 144-147.

Abdi, H., et al., The effects of body acupuncture on obesity: anthropometric parameters, lipid profile, and inflammatory and immunologic markers. The Scientific World Journal, 2012. 2012.

Ghayour-Mobarhan, M., et al., Dietary antioxidants and fat are associated with plasma antibody titers to heat shock proteins 60, 65, and 70 in subjects with dyslipidemia. The American journal of clinical nutrition, 2005. 81(5): p. 998-1004.

Pourghadamyari, H., et al., Serum antibody titers against heat shock protein 27 are associated with the severity of coronary artery disease. Cell Stress and Chaperones, 2011. 16(3): p. 309-316.

IssueVol 3, No 4 (Autumn 2017) QRcode
SectionOriginal Article(s)
Overweight Radiofrequency Ultrasound cavitation Hemoglobin; Hematocrit;

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How to Cite
Mohammadzadeh E, Arabpour Dahoue M, Sahebkar A, Kianoush T, Avan A, Nezafati P, Sadabadi F, Ghazizadeh H, Safar Soflaei S, Mohammadzadeh M, Nasrfard S, Safarian M, Norouzi A, Parizadeh seyed MR, Ghayour-Mobarhan M. Combined Radiofrequency and Ultrasonic Cavitation Therapy Does not Have Adverse Effects on Hematological and Liver Markers in Overweight Women. J Nutr Sci & Diet. 3(4):14-21.