The Effects of vitamin D supplementation on lipid and inflammatory profile of healthy adolescent boys
A randomized controlled trial
Abstract
Background: Deficiency of Vitamin D, an anti-inflammatory micronutrient with some favorable effects on lipid profiles, has been found to be highly prevalent in adolescents. We aimed to investigate the effect of a high-dose vitamin D supplementation on the lipid and inflammatory profiles of healthy adolescent boys.
Methods: In this randomized double-blind placebo-controlled trial, sixty healthy adolescent boys (age 17 yr) were recruited from one high school in Tehran, Iran and randomly assigned to two groups. The supplement group received vitamin D pearls at a dose of 50,000 IU monthly for 6 months and the other group was given placebo pearls for the same duration. Before and after the treatment, the serum levels of 25-hydroxy vitamin D (25(OH) D), parathyroid hormone (PTH), retinol, lead (Pb), the lipid profile and inflammatory biomarkers were measured and compared.
Results: Between groups statistical analysis showed that a high dose (50,000 IU/month) vitamin D significantly increased and decreased serum levels of vitaamin D and PTH, respectively (P < 0.05). No significant change was observed in serum levels of retinol and Pb. Between-group analysis revealed that the serum levels of TG and HDL decreased and increased, respectively (P < 0.05). Both the within- and between-group analysis showed that serum tumor necrosis factor receptor 2 (TNFR2) concentration declined while serum interleukin-10 (IL-10) increased in response to vitamin D supplementation (P < 0.05).
Conclusion: A high dose (50,000 IU/month) Vitamin D supplement seems to have some positive impact on serum vitamin D, lipid profile and inflammatory biomarkers in healthy adolescent boys.
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9. Pouraram H.; Djazayery, A.; Mohammad, K.; Parsaeian, M.; Abdollahi, Z.; Motlagh, A. D.; Djalali, M.; Khodaverdian, K.; Sotoudeh, G.; Yarparvar, A. Second National Integrated Micronutrient Survey in Iran: Study Design and Preliminary Findings. Archives of Iranian Medicine (AIM). 2018, 21(4).
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11. De Vita F.; Lauretani, F.; Bauer, J.; Bautmans, I.; Shardell, M.; Cherubini, A.; Bondi, G.; Zuliani, G.; Bandinelli, S.; Pedrazzoni, M. Relationship between vitamin D and inflammatory markers in older individuals. Age. 2014, 36(4): 9694.
12. Hirschler V.; Maccallini, G.; Sanchez, M. S.; Castaño, L.; Molinari, C.; Group, S. A. d. l. C. S. Improvement in high-density lipoprotein cholesterol levels in argentine Indian school children after vitamin D supplementation. Hormone research in paediatrics. 2013, 80(5): 335-42.
13. Feroze U.; Molnar, M. Z.; Dukkipati, R.; Kovesdy, C. P.; Kalantar-Zadeh, K. Insights into nutritional and inflammatory aspects of low parathyroid hormone in dialysis patients. Journal of Renal Nutrition. 2011, 21(1): 100-4.
14. Reid I. R. Effects of calcium supplementation on circulating lipids. Drugs & aging. 2004, 21(1): 7-17.
15. Kelishadi R.; Ardalan, G.; Motlagh, M. E.; Shariatinejad, K.; Heshmat, R.; Poursafa, P.; Fakhri, M.; Tajadini, M.; Taslimi, M. National report on the association of serum vitamin D with cardiometabolic risk factors in the pediatric population of the Middle East and North Africa (MENA): The CASPIAN-III Study. Nutrition. 2014, 30(1): 33-8.
16. Khayyatzadeh S. S.; Mirmoosavi, S. J.; Fazeli, M.; Abasalti, Z.; Avan, A.; Javandoost, A.; Rahmani, F.; Tayefi, M.; Hanachi, P.; Ferns, G. A. High-dose vitamin D supplementation is associated with an improvement in several cardio-metabolic risk factors in adolescent girls: a nine-week follow-up study. Annals of clinical biochemistry. 2018, 55(2): 227-35.
17. Tavakoli F.; Namakin, K.; Zardast, M. Vitamin D supplementation and high-density lipoprotein cholesterol: A study in healthy school children. Iranian journal of pediatrics. 2016, 26(4).
18. Muldowney S.; Kiely, M. Vitamin D and cardiometabolic health: a review of the evidence. Nutrition Research Reviews. 2011, 24(1): 1-20
19. Amer M.; Qayyum, R. Relation between serum 25-hydroxyvitamin D and C-reactive protein in asymptomatic adults (from the continuous National Health and Nutrition Examination Survey 2001 to 2006). Am J Cardiol. 2012, 109(2): 226-30.
20. Belenchia A. M.; Tosh, A. K.; Hillman, L. S.; Peterson, C. A. Correcting vitamin D insufficiency improves insulin sensitivity in obese adolescents: a randomized controlled trial. Am J Clin Nutr. 2013, 97(4): 774-81.
21. Cannell J. J.; Grant, W. B.; Holick, M. F. Vitamin D and inflammation. Dermatoendocrinol. 2014, 6(1): e983401.
22. Henriksen V. T.; Rogers, V. E.; Rasmussen, G. L.; Trawick, R. H.; Momberger, N. G.; Aguirre, D.; Barker, T. Pro-inflammatory cytokines mediate the decrease in serum 25(OH)D concentrations after total knee arthroplasty? Med Hypotheses. 2014, 82(2): 134-7.
23. Autier P.; Boniol, M.; Pizot, C.; Mullie, P. Vitamin D status and ill health: a systematic review. Lancet Diabetes Endocrinol. 2014, 2(1): 76-89.
24. Guillot X.; Semerano, L.; Saidenberg-Kermanac'h, N.; Falgarone, G.; Boissier, M. C. Vitamin D and inflammation. Joint Bone Spine. 2010, 77(6): 552-7.
25. Guo J.; Ma, Z.; Ma, Q.; Wu, Z.; Fan, P.; Zhou, X.; Chen, L.; Zhou, S.; Goltzman, D.; Miao, D.; Wu, E. 1, 25(OH)(2)D(3) inhibits hepatocellular carcinoma development through reducing secretion of inflammatory cytokines from immunocytes. Curr Med Chem. 2013, 20(33): 4131-41.
26. Aranow C. Vitamin D and the immune system. J Investig Med. 2011, 59(6): 881-6.
27. Wang Y.; Si, S.; Liu, J.; Wang, Z.; Jia, H.; Feng, K.; Sun, L.; Song, S. J. The Associations of Serum Lipids with Vitamin D Status. PLOS ONE. 2016, 11(10): e0165157.
28. Nouri Saeidlou S.; Vahabzadeh, D.; Babaei, F.; Vahabzadeh, Z. Seasonal variations of vitamin D and its relation to lipid profile in Iranian children and adults. Journal of health, population, and nutrition. 2017, 36(1): 21-.
29. Saedisomeolia A.; Taheri, E.; Djalali, M.; Moghadam, A. M.; Qorbani, M. Association between serum level of vitamin D and lipid profiles in type 2 diabetic patients in Iran. Journal of diabetes and metabolic disorders. 2014, 13(1): 7-.
30. Wang J. H.; Keisala, T.; Solakivi, T.; Minasyan, A.; Kalueff, A. V.; Tuohimaa, P. Serum cholesterol and expression of ApoAI, LXRbeta and SREBP2 in vitamin D receptor knock-out mice. J Steroid Biochem Mol Biol. 2009, 113(3-5): 222-6.
31. Christensen R.; Lorenzen, J. K.; Svith, C. R.; Bartels, E. M.; Melanson, E. L.; Saris, W. H.; Tremblay, A.; Astrup, A. Effect of calcium from dairy and dietary supplements on faecal fat excretion: a meta-analysis of randomized controlled trials. Obes Rev. 2009, 10(4): 475-86.
32. Ni Z.; Smogorzewski, M.; Massry, S. G. Effects of parathyroid hormone on cytosolic calcium of rat adipocytes. Endocrinology. 1994, 135(5): 1837-44.
2. Rosen C. J.; Adams, J. S.; Bikle, D. D.; Black, D. M.; Demay, M. B.; Manson, J. E.; Murad, M. H.; Kovacs, C. S. The nonskeletal effects of vitamin D: an Endocrine Society scientific statement. Endocrine reviews. 2012, 33(3): 456-92.
3. Rawal G.; Yadav, S.; Shokeen, P. Health and the vitamin D. Int J Health Sci Res. 2015, 5: 416-23.
4. Holick M. F.; Binkley, N. C.; Bischoff-Ferrari, H. A.; Gordon, C. M.; Hanley, D. A.; Heaney, R. P.; Murad, M. H.; Weaver, C. M. Evaluation, treatment, and prevention of vitamin D deficiency: an Endocrine Society clinical practice guideline. The Journal of Clinical Endocrinology & Metabolism. 2011, 96(7): 1911-30.
5. Green T.; Skeaff, C.; Rockell, J.; Venn, B.; Lambert, A.; Todd, J.; Khor, G.; Loh, S.; Muslimatun, S.; Agustina, R. Vitamin D status and its association with parathyroid hormone concentrations in women of child-bearing age living in Jakarta and Kuala Lumpur. European journal of clinical nutrition. 2008, 62(3): 373.
6. Al-Sadat N.; Majid, H. A.; Sim, P. Y.; Su, T. T.; Dahlui, M.; Bakar, M. F. A.; Dzaki, N.; Norbaya, S.; Murray, L.; Cantwell, M. M. Vitamin D deficiency in Malaysian adolescents aged 13 years: findings from the Malaysian Health and Adolescents Longitudinal Research Team study (MyHeARTs). BMJ open. 2016, 6(8): e010689.
7. Khalaji N.; Asadzadeh, S.; Neyestani, T.; Hajifaraji, M.; Omidvar, N.; Shariatzadeh, N.; Kalayi, A.; Nikooyeh, B.; Mohammadi, M. High prevalence of vitamin D deficiency in school age children in Tehran, 2008: a red alert. Iranian Journal of Nutrition Sciences & Food Technology. 2013, 7(5): 389-98.
8. Ataie-Jafari A.; Qorbani, M.; Heshmat, R.; Ardalan, G.; Motlagh, M. E.; Asayesh, H.; Arzaghi, S. M.; Tajadini, M. H.; Nejatinamini, S.; Poursafa, P. The association of vitamin D deficiency with psychiatric distress and violence behaviors in Iranian adolescents: the CASPIAN-III study. Journal of Diabetes & Metabolic Disorders. 2015, 14(1): 62.
9. Pouraram H.; Djazayery, A.; Mohammad, K.; Parsaeian, M.; Abdollahi, Z.; Motlagh, A. D.; Djalali, M.; Khodaverdian, K.; Sotoudeh, G.; Yarparvar, A. Second National Integrated Micronutrient Survey in Iran: Study Design and Preliminary Findings. Archives of Iranian Medicine (AIM). 2018, 21(4).
10. Chaudhuri J. R.; Mridula, K. R.; Anamika, A.; Boddu, D. B.; Misra, P. K.; Lingaiah, A.; Balaraju, B.; Bandaru, V. S. Deficiency of 25-hydroxyvitamin d and dyslipidemia in Indian subjects. Journal of lipids. 2013, 2013.
11. De Vita F.; Lauretani, F.; Bauer, J.; Bautmans, I.; Shardell, M.; Cherubini, A.; Bondi, G.; Zuliani, G.; Bandinelli, S.; Pedrazzoni, M. Relationship between vitamin D and inflammatory markers in older individuals. Age. 2014, 36(4): 9694.
12. Hirschler V.; Maccallini, G.; Sanchez, M. S.; Castaño, L.; Molinari, C.; Group, S. A. d. l. C. S. Improvement in high-density lipoprotein cholesterol levels in argentine Indian school children after vitamin D supplementation. Hormone research in paediatrics. 2013, 80(5): 335-42.
13. Feroze U.; Molnar, M. Z.; Dukkipati, R.; Kovesdy, C. P.; Kalantar-Zadeh, K. Insights into nutritional and inflammatory aspects of low parathyroid hormone in dialysis patients. Journal of Renal Nutrition. 2011, 21(1): 100-4.
14. Reid I. R. Effects of calcium supplementation on circulating lipids. Drugs & aging. 2004, 21(1): 7-17.
15. Kelishadi R.; Ardalan, G.; Motlagh, M. E.; Shariatinejad, K.; Heshmat, R.; Poursafa, P.; Fakhri, M.; Tajadini, M.; Taslimi, M. National report on the association of serum vitamin D with cardiometabolic risk factors in the pediatric population of the Middle East and North Africa (MENA): The CASPIAN-III Study. Nutrition. 2014, 30(1): 33-8.
16. Khayyatzadeh S. S.; Mirmoosavi, S. J.; Fazeli, M.; Abasalti, Z.; Avan, A.; Javandoost, A.; Rahmani, F.; Tayefi, M.; Hanachi, P.; Ferns, G. A. High-dose vitamin D supplementation is associated with an improvement in several cardio-metabolic risk factors in adolescent girls: a nine-week follow-up study. Annals of clinical biochemistry. 2018, 55(2): 227-35.
17. Tavakoli F.; Namakin, K.; Zardast, M. Vitamin D supplementation and high-density lipoprotein cholesterol: A study in healthy school children. Iranian journal of pediatrics. 2016, 26(4).
18. Muldowney S.; Kiely, M. Vitamin D and cardiometabolic health: a review of the evidence. Nutrition Research Reviews. 2011, 24(1): 1-20
19. Amer M.; Qayyum, R. Relation between serum 25-hydroxyvitamin D and C-reactive protein in asymptomatic adults (from the continuous National Health and Nutrition Examination Survey 2001 to 2006). Am J Cardiol. 2012, 109(2): 226-30.
20. Belenchia A. M.; Tosh, A. K.; Hillman, L. S.; Peterson, C. A. Correcting vitamin D insufficiency improves insulin sensitivity in obese adolescents: a randomized controlled trial. Am J Clin Nutr. 2013, 97(4): 774-81.
21. Cannell J. J.; Grant, W. B.; Holick, M. F. Vitamin D and inflammation. Dermatoendocrinol. 2014, 6(1): e983401.
22. Henriksen V. T.; Rogers, V. E.; Rasmussen, G. L.; Trawick, R. H.; Momberger, N. G.; Aguirre, D.; Barker, T. Pro-inflammatory cytokines mediate the decrease in serum 25(OH)D concentrations after total knee arthroplasty? Med Hypotheses. 2014, 82(2): 134-7.
23. Autier P.; Boniol, M.; Pizot, C.; Mullie, P. Vitamin D status and ill health: a systematic review. Lancet Diabetes Endocrinol. 2014, 2(1): 76-89.
24. Guillot X.; Semerano, L.; Saidenberg-Kermanac'h, N.; Falgarone, G.; Boissier, M. C. Vitamin D and inflammation. Joint Bone Spine. 2010, 77(6): 552-7.
25. Guo J.; Ma, Z.; Ma, Q.; Wu, Z.; Fan, P.; Zhou, X.; Chen, L.; Zhou, S.; Goltzman, D.; Miao, D.; Wu, E. 1, 25(OH)(2)D(3) inhibits hepatocellular carcinoma development through reducing secretion of inflammatory cytokines from immunocytes. Curr Med Chem. 2013, 20(33): 4131-41.
26. Aranow C. Vitamin D and the immune system. J Investig Med. 2011, 59(6): 881-6.
27. Wang Y.; Si, S.; Liu, J.; Wang, Z.; Jia, H.; Feng, K.; Sun, L.; Song, S. J. The Associations of Serum Lipids with Vitamin D Status. PLOS ONE. 2016, 11(10): e0165157.
28. Nouri Saeidlou S.; Vahabzadeh, D.; Babaei, F.; Vahabzadeh, Z. Seasonal variations of vitamin D and its relation to lipid profile in Iranian children and adults. Journal of health, population, and nutrition. 2017, 36(1): 21-.
29. Saedisomeolia A.; Taheri, E.; Djalali, M.; Moghadam, A. M.; Qorbani, M. Association between serum level of vitamin D and lipid profiles in type 2 diabetic patients in Iran. Journal of diabetes and metabolic disorders. 2014, 13(1): 7-.
30. Wang J. H.; Keisala, T.; Solakivi, T.; Minasyan, A.; Kalueff, A. V.; Tuohimaa, P. Serum cholesterol and expression of ApoAI, LXRbeta and SREBP2 in vitamin D receptor knock-out mice. J Steroid Biochem Mol Biol. 2009, 113(3-5): 222-6.
31. Christensen R.; Lorenzen, J. K.; Svith, C. R.; Bartels, E. M.; Melanson, E. L.; Saris, W. H.; Tremblay, A.; Astrup, A. Effect of calcium from dairy and dietary supplements on faecal fat excretion: a meta-analysis of randomized controlled trials. Obes Rev. 2009, 10(4): 475-86.
32. Ni Z.; Smogorzewski, M.; Massry, S. G. Effects of parathyroid hormone on cytosolic calcium of rat adipocytes. Endocrinology. 1994, 135(5): 1837-44.
| Issue | Vol 5, No 3/4 (Summer/Autumn 2019) | |
| Section | Original Article(s) | |
| Keywords | ||
| Adolescent Inflammation Lipid profile PTH Vitamin D | ||
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How to Cite
1.
Yarparvar A. The Effects of vitamin D supplementation on lipid and inflammatory profile of healthy adolescent boys. J Nutr Sci & Diet. 2021;5(3-4).
