Interactive Effects of Endurance Training and Selenium Consumption on the Intrinsic Apoptosis Pathway in the Liver Tissue of Cadmium-Exposed Rats
Background: Cadmium can damage various tissues, including the liver, by fragmenting DNA of cells through increasing free radicals and reactive oxygen species (ROS) as well as inhibition of apoptotic suppressor proteins.
Objectives: The purpose of this study was to investigate the interactive effects of endurance training and selenium consumption on the intrinsic apoptosis pathway in the liver tissue ofcadmium-exposed rats.
Methods: In this experimental study, 30 rats were selected and divided into six groups of five subjects: (1) control, (2) selenium consumption, (3) high intensity interval training (HIIT), (4) continuous training, (5) HIIT with selenium, and (6) continuous trainingwithselenium.All rats peritoneally received 2 mg/kg of body weight cadmium for eight weeks. Rats in groups 2, 5 and 6 received0.23 mg/kg of selenium daily.Also, groups 3 to6 performed three sessionsof continuous and high intensity interval training for eight weeks. Two-way ANOVA along with Bonferroni's pairwise comparison test was used to analyze the findings of the research (P≤0.05).
Results: Training increased Bcl-2 in the liver tissue of cadmium-exposed rats (p≤0.05);also, HIIT had a greater effect on Bcl-2 than continuous training (p ≤0.05).Selenium consumption reduced Bax and increased Bcl-2 in the liver tissue of cadmium-exposed rats (p≤0.05); on the other hand, exercise trainingand selenium consumption had interactive effects on decreasing caspase-9 and Bax and increasing Bcl-2 expression in the liver tissue of rats exposed to cadmium (p ≤0.05).
Conclusions: Endurance training along with selenium consumption seems to have interactive effects on improving the process of liver apoptosis in rats exposed to cadmium.
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