Omega-3 Fatty Acid Modifies Serum HSP 27 in Patients with Cardiovascular Disease: Randomized Double‐Blind Placebo-Controlled Trial
-
Simin Samavat
,
Mahmoud Djalali
,
Ebrahim Nematipour
,
Mohammad Reza Eshraghian
,
Mahnaz Zarei
,
Sanaz Gholamhoseini
,
Mohammad Hassan Javanbakht
Abstract
Background: Heat shock proteins 27 (HSP-27) can play an important role in the pathogenesis of atherosclerosis. Omega-3 fatty acids include anti-inflammatory eicosanoids and contribute to the primary and secondary prevention of cardiovascular disease (CVD). The purpose of the current study is to assess the effect of omega-3 on serum HSP-27 in patient with atherosclerosis.
Methods: In a double‐blind placebo-controlled trial study, 42 patients were selected from Tehran Heart Hospital. Both intervention (n=21) and placebo (n=21) groups were men with cardiovascular disease (based on Angiography). The intervention group received omega-3 supplement and placebo group took edible paraffin. Dietary intakes, physical activity level, anthropometric parameters and body composition, were measured. Serum HSP-27 concentration was determined using ELISA.
Results: After two months, change differences in HSP-27 between two groups was statistically significant (p=0.001). This difference remained significant even after adjusting for serum LDL concentration (p=0.002).
Conclusions: Our results showed that taking omega-3 fatty acids can ameliorate serum HSP-27 as inflammatory parameters. Our results suggest more investigation to assess the pathway omega-3 leads to lower incidence of CVD.
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10. Lavie CJ, Milani RV, Mehra MR, Ventura HO.Omega-3 polyunsaturated fatty acids andcardiovascular diseases. J Am Coll Cardiol.2009;54(7):585-94.
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14. Hightower LE, Guidon PT. Selective release fromcultured mammalian cells of heat‐shock (stress)proteins that resemble glia‐axon transfer proteins.J Cell Physiol. 1989;138(2):257-66.
15. Asea A. Stress proteins and initiation of immuneresponse: chaperokine activity of hsp72. ExercImmunol Rev. 2005;11:34.
16. Wick G, Knoflach M, Xu Q. Autoimmune andinflammatory mechanisms in atherosclerosis.Annu Rev Immunol. 2004;22:361-403.
17. Arbour NC, Lorenz E, Schutte BC, Zabner J,Kline JN, Jones M, et al. TLR4 mutations areassociated with endotoxin hyporesponsiveness inhumans. Nat Genet. 2000;25(2):187-91.
18. Tytell M, Greenberg S, Lasek R. Heat shock-likeprotein is transferred from glia to axon. Brain Res.1986;363(1):161-4.
19. Park HK, Park E-C, Bae SW, Park MY, Kim SW,Yoo HS, et al. Expression of heat shock protein27 in human atherosclerotic plaques and increasedplasma level of heat shock protein 27 in patientswith acute coronary syndrome. Circulation.2006;114(9):886-93.
20. Heidari-Bakavoli AR, Sahebkar A, Mobara N,Moohebati M, Tavallaie S, Rahsepar AA, et al.Changes in plasma level of heat shock protein 27after acute coronary syndrome. Angiology.2012;63(1):12-6.
21. Martin-Ventura JL, Duran MC, Blanco-Colio LM,Meilhac O, Leclercq A, Michel J-B, et al.Identification by a differential proteomic approachof heat shock protein 27 as a potential marker ofatherosclerosis. Circulation. 2004;110(15):2216-9.
22. Wick G. The Heat Is on Heat-Shock Proteins andAtherosclerosis. Circulation. 2006;114(9):870-2.
23. Préville X, Salvemini F, Giraud S, Chaufour S,Paul C, Stepien G, et al. Mammalian small stressproteins protect against oxidative stress throughtheir ability to increase glucose-6-phosphatedehydrogenase activity and by maintainingoptimal cellular detoxifying machinery. Exp CellRes. 1999;247(1):61-78.
24. Arrigo A-P, Virot S, Chaufour S, Firdaus W,Kretz-Remy C, Diaz-Latoud C. Hsp27consolidates intracellular redox homeostasis byupholding glutathione in its reduced form and bydecreasing iron intracellular levels. AntioxidRedox Signal. 2005;7(3-4):414-22.
25. Burut DFP, Borai A, Livingstone C, Ferns G.Serum heat shock protein 27 antigen and antibodylevels appear to be related to the macrovascularcomplications associated with insulin resistance: apilot study. Cell Stress Chaperones.
2010;15(4):379-86.
26. Salinthone S, Ba M, Hanson L, Martin JL,Halayko AJ, Gerthoffer WT. Overexpression ofhuman Hsp27 inhibits serum-induced proliferationin airway smooth muscle myocytes and confersresistance to hydrogen peroxide cytotoxicity. AmJ Physiol Lung Cell Mol Physiol.2007;293(5):L1194-L207.
27. Rogalla T, Ehrnsperger M, Preville X, KotlyarovA, Lutsch G, Ducasse C, et al. Regulation ofHsp27 oligomerization, chaperone function, andprotective activity against oxidative stress/tumornecrosis factor α by phosphorylation. J BiolChem. 1999;274(27):18947-56.
28. Robinson AA, Dunn MJ, McCormack A, dosRemedios C, Rose ML. Protective effect ofphosphorylated Hsp27 in coronary arteriesthrough actin stabilization. J Mol Cell Cardiol.2010;49(3):370-9.
29. Ebrahimi M, Ghayour-Mobarhan M, Rezaiean S,Hoseini M, Parizade SMR, Farhoudi F, et al.Omega-3 fatty acid supplements improve thecardiovascular risk profile of subjects withmetabolic syndrome, including markers ofinflammation and auto-immunity. Acta Cardiol.2009;64(3):321-7.
2. Epstein FH, Ross R. Atherosclerosis—aninflammatory disease. N Engl J Med.1999;340(2):115-26.
3. Xu Q. Role of heat shock proteins inatherosclerosis. Arterioscler Thromb Vasc Biol.2002;22(10):1547-59.
4. Hu Z, Yang B, Lu W, Zhou W, Zeng L, Li T, etal. HSPB2/MKBP, a novel and unique member ofthe small heat‐shock protein family. J NeurosciRes. 2008;86(10):2125-33.
5. Ghayour-Mobarhan M, Saber H, Ferns GA. Thepotential role of heat shock protein 27 incardiovascular disease. Clin Chim Acta.2012;413(1):15-24.
6. Józefowicz-Okonkwo G, Wierzbowska-Drabik K,Kasielski M, Trzos E, Goraca A, Nowak D, et al.Is Hsp27 a marker of myocardial ischaemia?Kardiologia Polska. 2009;67(9):947-52.
7. Connor WE. Importance of n− 3 fatty acids inhealth and disease. Am J Clin Nutr.2000;71(1):171S-5S.
8. Geleijnse JM, Giltay EJ, Grobbee DE, DondersAR, Kok FJ. Blood pressure response to fish oilsupplementation: metaregression analysis ofrandomized trials. J Hypertens.2002;20(8):1493-9.
9. Aarsetoey H, Grundt H, Nygaard O, Nilsen DW.The role of long-chained marine N-3polyunsaturated fatty acids in cardiovasculardisease. Cardiology Research and Practice.2012;2012:303456.
10. Lavie CJ, Milani RV, Mehra MR, Ventura HO.Omega-3 polyunsaturated fatty acids andcardiovascular diseases. J Am Coll Cardiol.2009;54(7):585-94.
11. Saravanan P, Davidson NC, Schmidt EB, CalderPC. Cardiovascular effects of marine omega-3fatty acids. The Lancet. 2010;376(9740):540-50.
12. Organization WH. Waist Circumference andWaist-Hip Ratio Report of a WHO ExpertConsultation. December 2008.
13. Sangster TA, Salathia N, Undurraga S, Milo R,Schellenberg K, Lindquist S, et al. HSP90 affectsthe expression of genetic variation anddevelopmental stability in quantitative traits. ProcNatl Acad Sci. 2008;105(8):2963-8.
14. Hightower LE, Guidon PT. Selective release fromcultured mammalian cells of heat‐shock (stress)proteins that resemble glia‐axon transfer proteins.J Cell Physiol. 1989;138(2):257-66.
15. Asea A. Stress proteins and initiation of immuneresponse: chaperokine activity of hsp72. ExercImmunol Rev. 2005;11:34.
16. Wick G, Knoflach M, Xu Q. Autoimmune andinflammatory mechanisms in atherosclerosis.Annu Rev Immunol. 2004;22:361-403.
17. Arbour NC, Lorenz E, Schutte BC, Zabner J,Kline JN, Jones M, et al. TLR4 mutations areassociated with endotoxin hyporesponsiveness inhumans. Nat Genet. 2000;25(2):187-91.
18. Tytell M, Greenberg S, Lasek R. Heat shock-likeprotein is transferred from glia to axon. Brain Res.1986;363(1):161-4.
19. Park HK, Park E-C, Bae SW, Park MY, Kim SW,Yoo HS, et al. Expression of heat shock protein27 in human atherosclerotic plaques and increasedplasma level of heat shock protein 27 in patientswith acute coronary syndrome. Circulation.2006;114(9):886-93.
20. Heidari-Bakavoli AR, Sahebkar A, Mobara N,Moohebati M, Tavallaie S, Rahsepar AA, et al.Changes in plasma level of heat shock protein 27after acute coronary syndrome. Angiology.2012;63(1):12-6.
21. Martin-Ventura JL, Duran MC, Blanco-Colio LM,Meilhac O, Leclercq A, Michel J-B, et al.Identification by a differential proteomic approachof heat shock protein 27 as a potential marker ofatherosclerosis. Circulation. 2004;110(15):2216-9.
22. Wick G. The Heat Is on Heat-Shock Proteins andAtherosclerosis. Circulation. 2006;114(9):870-2.
23. Préville X, Salvemini F, Giraud S, Chaufour S,Paul C, Stepien G, et al. Mammalian small stressproteins protect against oxidative stress throughtheir ability to increase glucose-6-phosphatedehydrogenase activity and by maintainingoptimal cellular detoxifying machinery. Exp CellRes. 1999;247(1):61-78.
24. Arrigo A-P, Virot S, Chaufour S, Firdaus W,Kretz-Remy C, Diaz-Latoud C. Hsp27consolidates intracellular redox homeostasis byupholding glutathione in its reduced form and bydecreasing iron intracellular levels. AntioxidRedox Signal. 2005;7(3-4):414-22.
25. Burut DFP, Borai A, Livingstone C, Ferns G.Serum heat shock protein 27 antigen and antibodylevels appear to be related to the macrovascularcomplications associated with insulin resistance: apilot study. Cell Stress Chaperones.
2010;15(4):379-86.
26. Salinthone S, Ba M, Hanson L, Martin JL,Halayko AJ, Gerthoffer WT. Overexpression ofhuman Hsp27 inhibits serum-induced proliferationin airway smooth muscle myocytes and confersresistance to hydrogen peroxide cytotoxicity. AmJ Physiol Lung Cell Mol Physiol.2007;293(5):L1194-L207.
27. Rogalla T, Ehrnsperger M, Preville X, KotlyarovA, Lutsch G, Ducasse C, et al. Regulation ofHsp27 oligomerization, chaperone function, andprotective activity against oxidative stress/tumornecrosis factor α by phosphorylation. J BiolChem. 1999;274(27):18947-56.
28. Robinson AA, Dunn MJ, McCormack A, dosRemedios C, Rose ML. Protective effect ofphosphorylated Hsp27 in coronary arteriesthrough actin stabilization. J Mol Cell Cardiol.2010;49(3):370-9.
29. Ebrahimi M, Ghayour-Mobarhan M, Rezaiean S,Hoseini M, Parizade SMR, Farhoudi F, et al.Omega-3 fatty acid supplements improve thecardiovascular risk profile of subjects withmetabolic syndrome, including markers ofinflammation and auto-immunity. Acta Cardiol.2009;64(3):321-7.
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| Issue | Vol 1, No 4 (Autumn 2015) | |
| Section | Original Article(s) | |
| Keywords | ||
| HSP-27 cardiovascular disease Omega-3 fatty acid. | ||
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How to Cite
1.
Samavat S, Djalali M, Nematipour E, Eshraghian MR, Zarei M, Gholamhoseini S, Javanbakht MH. Omega-3 Fatty Acid Modifies Serum HSP 27 in Patients with Cardiovascular Disease: Randomized Double‐Blind Placebo-Controlled Trial. J Nutr Sci & Diet. 2016;1(4):188-193.
