Tehran University of Medical Sciences Journal of Nutritional Sciences and Dietetics 1 4 2015 11 02 181 187 EN Simin Haghravan Department of nutritional science and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. Seyedali Keshavarz Department of nutritional science and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. Seyede-elham Sharafi Department of psychiatric, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran Zahra Alizadeh Sports and Exercise Medicine Sports Medicine Research Center, Faculty of Medicine Tehran University of Medical Sciences, Tehran, Iran. 2015 11 23 2016 04 02 Background: Anxiety, stress and depression are still one of the common chronic diseases among Iranian population especially among women in the age of 15-29 years. Associations between depressed mood and lifestyle are well known. The aim of this study was to investigate the effect of omega-3 poly unsaturated fatty acids (PUFA)s supplementation with lifestyle modification on depression and anxiety in overweight women. Methods: Thirty eight women aged between 20-45 years; body mass index (BMI) equal 25-30; having sedentary lifestyle without any known metabolic, cardiovascular or pulmonary diseases or taking any medication which affect heart rate, blood pressure or exercise capacity were included. Patients randomly divided into two experimental groups. Group 1 received omega-3 capsules plus aerobic exercise and nutrition education classes. Group 2 received placebo capsules plus aerobic exercise and nutrition education classes. All of the subjects in group 1were asked to take one capsule/day, for 8 weeks. The depression level was measured using the Hospital Anxiety and Depression Scale. Results: There were no significant differences between anxiety and educational level, depression and educational level in overweight women at baseline, 4 and 8 weeks of the study. In addition, no significant correlation was found between changes observed on anxiety and depression extent and different occupations among volunteer women at baseline, 4 and 8 weeks of the study. Omega-3 capsules significantly decreased anxiety and depression compared to control group (P=0.032). Conclusion: Omega-3 fatty acids supplementation may have beneficial effects onanxiety and depression in overweight women that is not related to lifestylemodification. Further randomized controlled trials with large sample size are needed toconfirm these results https://jnsd.tums.ac.ir/index.php/jnsd/article/view/27 https://jnsd.tums.ac.ir/index.php/jnsd/article/download/27/38