Tehran University of Medical Sciences Journal of Nutritional Sciences and Dietetics 2 2 2017 08 01 111 111 EN Samira Ebrahimof Nutrition and Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Sakineh Shab-bidar Department of Community Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran Marjan Zarif Yeganeh Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Pooneh Angoorani National Nutrition and Food Technology Research Institute, Faculty of Nutrition and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran Somayeh Abedini National Nutrition and Food Technology Research Institute, Faculty of Nutrition and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran Farzaneh Jahangir School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran Maryam-Sadat Daneshpour Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mehdi Hedayati Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2017 06 23 2017 06 25 Objective: The possible role of vitamin D receptor (VDR) variants in determining cardiovascular risk factors has been suggested previously. The purpose of the present study was to evaluate the associations of variants of vitamin D receptor (VDR) gene with metabolic profile in Iranian overweight women with hypovitaminosis D. Materials and Methods: Variants in VDR gene including FokI (rs2228570), BsmI (rs1544410) and rs757343 were detected using PCR-sequencing. Metabolic Profile including fasting plasma glucose, total cholesterol, LDL-C, HDL-C, and triacylglycerol was assessed using commercial kits. Circulating 25 (OH) D was measured using ELISA kits. Fat mass and visceral fat were measured using Bio Impedance Analysis. Results: A sample of 123 overweight women with hypovitaminosis D (25 (OH) D<75nmol/L) was studied. The mean 25 (OH) D level was 28.7±17.0nmol/L. The FokI polymorphism was significantly associated with total cholesterol (p=0.03) and LDL-C (p=0.01). Carriers of the CC genotype of the FokI polymorphism had lower total cholesterol (162.9±30.4 vs. 176.1±32.4, p=0. 02) and LDL-C (98.0±26.0 vs. 110.6±28.5, p=0. 01) compared with carriers of CT+TT genotype. Regression analysis revealed that the FokI variant and fat mass explained 18% of the variance in total cholesterol. Regarding LDL-C, FokI variant explained 21% of the variance in LDL-C. None of the studied polymorphisms were significantly associated with adiposity phenotypes (p>0.05). Also, adiposity phenotype measures were not significantly different in the genotype groups of the studied SNPs (p>0.5). Conclusions: The present results indicate that the FokI polymorphism is significantly associated with total cholesterol and LDL-C in Iranian overweight women with hypovitaminosis D.   https://jnsd.tums.ac.ir/index.php/jnsd/article/view/111 https://jnsd.tums.ac.ir/index.php/jnsd/article/download/111/148